Enzyme-loaded nanovesicles

Nanovesicles are ideal vehicles for many biomolecules, including enzymes. and might be useful in the treatment of different diseases, such as lysosomal storage disorders (LSD) or severe combined immunodeficiency (SCID). However, the development of such treatments requires a fine understanding of their synthetic processes and their biological impact.
To ensure good efficacy, it is therefore necessary to have a well-defined nanovesicle. For this reason, it is crucial to physicochemically characterize the enzyme-loaded nanovesicles, as these results will have an impact on the efficacy of this treatment. Once the physicochemical parameters have been studied, the functionality of these systems needs to be investigated using appropriate in vivo models.

Description

In this cutting-edge solution, we offer the possibility of producing enzyme-loaded nanovesicles and their full evaluation. The enzyme-loaded nanovesicles will be prepared using the DELOS-SUSP method, which allows the preparation of cholesterol-rich nanovesicles encapsulating some biological and non-biological molecules. The first analysis to perform is the physicochemical characterization, which includes the following characteristics: hydrodynamic diameter, polydispersity index, apparent zeta-potential, particle concentration, colloidal stability, etc. Afterwards, their functionality (enzymatic activity, in vitro and in vivo efficacy) and safety profile (toxicology and immunogenicity) will be tested using relevant animal models.
These properties are crucial to understand the behavior and efficacy of the enzyme-loaded nanovesicles.

More info: https://www.nanbiosis.es/enzyme-loaded-nanovesicles/