CLICK’N CUT

Universal and robust tool to purify tag-less proteins in a one-step process based on intein affinity. Suitable for every step of product and process development: R&D, preclinical, clinical and industrial manufacturing.

✓ Universal system to purify any Protein Of Interest (POI)

✓ High purity(>95%)

✓ Fast(5 hours to pure, tag-less POI)

✓ Simple: cleavage is triggered on column by a pH shift at physiological conditions

✓ Compatible with common expression host and buffers

✓ Generates a clean cut with no extra residues

✓ No premature cleavage during protein expression

✓ Cleavage takes place regardless of the identity of the first POI’s aminoacid