Mesoporous silica nanoparticles for immunoglobulin purification

Currently, commercial systems for the purification of immunoglobulin G (IgG) use G-protein bound particles or resins with inunoglobulin binding capacity.

However, one of the main problems in affinity purification is the performance that the resins or particles offer to the user. Currently, commercial products show binding capacity ranging from 6 μg Ig/mg of material to 125 μg IgG/mg of material.

To improve this performance, the research group has developed protein G-bonded mesoporous silica particles with a given particle and pore size that allow increasing the binding capacity of immunoglobulins to the particles.

Tests have shown an IgG uptake capacity of between 460-800 μg of IgG per mg of nanoparticles, representing at least a 4-fold improvement over the best performing commercial product.

The production system also allows obtaining nanoparticles of highly homogeneous particle size and pore size.