Mass Spetrometry Metabolomics: Bioactive Metabolites and Host-Microbiota Interactions

We offer advanced targeted and untargeted mass spectrometry–based metabolomics built around state-of-the-art UHPLC–high-resolution mass spectrometry with ion mobility separation (timsTOF HT, Bruker). Our platform enables comprehensive, high-sensitivity profiling of low-molecular-weight metabolites across complex biological matrices, supporting research on bioactive metabolites, microbial metabolism, and host–microbiota interactions.

Our expertise covers the complete metabolomics workflow, including standardized sample preparation and analysis of microbial cultures, cell lysates, and host-derived samples such as mouse and human feces, serum, and urine. We apply complementary chromatographic strategies (reverse-phase, HILIC, and derivatization-based methods) to maximize metabolome coverage. Data analysis integrates commercail, open-source, and in-house software and resources, covering tools for chemical space visualization and metadata mapping, metabolite annotation with large spectral libraries and in silico tools, statistical approaches for cohort-level comparisons and biomarker discovery, and repository-scale data exploration.

A key strength is the tight integration of metabolomics with biosynthesis, genome mining, and functional interpretation. We link metabolomic profiles to microbial gene clusters, metabolic pathways, and biological activity using differential metabolomics, stable isotope labeling, and genetic and biochemical validation. Our research includes discovery and characterization of novel bioactive microbial metabolites, with relevance to antimicrobial resistance and host–microbe communication, supported by unique gnotobiotic animal models.