Increasing the Effectiveness of Antitumor Drugs by Increasing Their Binding to Tumor Tissue Previously Irradiated with Non-Ionizing Microwaves

DNA released from sarcoma 45 tumor (DNAt) and healthy rats (DNAh) in water-saline solution was irradiated during 90 min. The absorption spectra of non-irradiated and irradiated complexes of DNA with anti-tumors drugs doxorubicin (DX) and netropsin (NT) were obtained by spectroscopic method. From the absorption spectra, binding constants К have been calculated. Results: With irradiated tumor DNA, the antitumor drug forms more stable complexes, the binding constant with DX and NT is almost an order of magnitude higher than for non-irradiated DNA, i.e. the same antitumor effect can be expected with much lower doses of drugs, which is actually equivalent to increasing their effectiveness.  Description of the Project: It is necessary to compare the dynamics of changes in tumor growth in three groups of animals: in the control group without radiation, where animals receive a regular course of treatment, and in two (or more) experimental ones, where they receive lower doses and are simultaneously exposed to radiation. If the therapeutic effect is the same in all groups, then this will confirm an increase in the degree of binding of the drug to the irradiated tumor tissue, which is important from the point of view of using gentle methods of treating patients (some patients are forced to interrupt treatment due to their intolerance) and reducing the costs associated with the purchase of expensive drugs (the dosage of drugs is significantly reduced. My role in the consortium: To provide the radio-physical part of the experiment, i.e. to irradiate the studied samples with low-intensity microwaves at selective (bio-resonance) frequencies according to the methodology developed by us. Тype of partner we are looking for: We are looking for a partner who could measure the coefficient (constant) of binding of an antitumor drug to the affected tissue after its irradiation with microwaves.