Non-Enzymatic Chemical Modification and Highly Sensitive Fluorescence Dye for Dranscription/Translation Detection

1.Building-up of Nucleic Acids in Cells; Efficient intracellular delivery and low side effect of RNA drugs

Introduction of nucleic acid strands, such as those constituting siRNA, into cells and chemical binding of the strand enables to construct larger functional nucleic acids within cells

Advantage of enhancing membrane permeability and avoiding immune activation.

2.Flexible DNA sticky end formation technology Three technologies can be selected depending on the application

Three types of chemical cohesive‑end formation technologies enable; 1.more flexible sequence design, 2.highly-efficient DNA ligation, 3.formation of a cohesive end on only one terminus

Useful for construction of genetically modified vectors.

3.Non-enzymatic oligonucleotide ligation method; Chemical synthesis of DNA/RNA with repetitive sequences

An organic chemical method for nucleic acid strand ligation employing a phosphorothioate-containing nucleic acid strand together with an electrophilic reagent

Enables the low-cost production of various functional nucleic acids and proteins

4.Nucleic acid probe; Imaging of mRNA, DNA dynamics in a living cell

Fluorescence emission intensity can be maximized by arranging multiple scaffold sequences containing fluorescent molecule-binding aptamers in tandem using a unique linker sequence, "Romanesco".

“ChrocodiLE” protein capable of selectively and sequence-independently binding to DNA of open chromatin with minimal steric hindrance was created. Fluorescent protein emits light upon binding of ChrocodiLE to DNA.