HNN3.0

Project cooperationUpdated on 12 December 2025

LNP delivery with barcoded mRNA

Professor at Ghent University

Ghent, Belgium

About

We offer expertise in lipid nanoparticle (LNP) delivery using barcoded mRNA payloads to enable high-throughput, quantitative evaluation of mRNA delivery and expression. By embedding short, inert nucleotide barcodes within the mRNA (typically in the 3′ UTR), multiple LNP formulations can be administered simultaneously and resolved post-delivery by sequencing.

This approach allows researchers to directly link LNP composition to biodistribution, cell-type uptake, and functional expression using bulk or single-cell RNA sequencing. It supports parallel screening of large LNP libraries in a single experiment, reducing time, cost, and biological variability compared with one-by-one testing.

Our knowledge covers library design, LNP formulation strategies, in vivo and in vitro delivery, and sequencing-based analysis (bulk, single-cell, and spatial) pipelines. This enables rational optimization of LNPs for specific tissues or cell types and accelerates development of mRNA vaccines, gene regulation tools, and therapeutic candidates.

Topic

  • DESTINATION 5: HORIZON-HLTH-2026-01-TOOL-07: Establishing a European network of Centres of Excellence (CoEs) for Advanced Therapies Medicinal Products (ATMPs)

Type

  • Partner seeks Consortium/Coordinator

Organisation

Ghent University

University

Ghent, Belgium

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