B-cell mediated immune responses in health and disease.

Our group has extensive expertise in studying the development and regulation of antibody-secreting cells, germinal center and memory B cells using mouse models of immunization, parasitic infection, and allergic inflammation. We employ a broad range of advanced tools and approaches, including in vivo CRISPR/Cas9 screens, in vitro B cell activation assays, sgRNA-mediated gene inactivation in naive and effector B cells, multicolor flow cytometry, and genetically engineered mouse models for targeted gene deletion. Additionally, we integrate transcriptomic and epigenetic profiling, and collaborate with computational scientists for high-dimensional data analysis to dissect molecular pathways controlling B cell differentiation and function. Our work combines mechanistic studies with translational insights to better understand humoral immunity in health and disease.