Effects of emerging chemicals on sole (Solea senegalensis)

Authors: Ketil Hylland, Joachim Sturve_, Xabi Lekube_*

Affiliations: University of Oslo/Institute of Marine Research (NO), University of Gothenburg (SE), *Plentzia Marine Station (ES)

Abstract: The experiment evaluated sublethal impacts of three CECs: 3,4,5-trimethylphenyl methylcarbamate (trimethacarb, pesticide), oxfendazole (antihelmintic pharmaceutical), octinoxate (sunscreen), singly and in a combined exposure. In addition to providing important information about the potential environmental toxicity of the above substances, the project aims to identify potential biomarkers to be used in environmental monitoring. Analyses are currently underway, including existing biomarkers such as lysosomal stability, micronucleus frequency, plasma thyroid and lysozyme concentration. Novel biomarkers will be identified using proteomics analyses of plasma and heart. The fish were be exposed for 14 and 42 days. All individuals were labelled using pit-tag, making it possible to follow individual growth through the exposure period and keep the fish in mixed exposure groups. There was no chemical-induced mortality or difference in growth or condition in the exposure groups. Preliminary data indicate increased level of precursor red blood cells in some groups and differences in heart proteome. The remaining analyses are being performed at this time.